horseradish peroxidase conjugated goat anti rat igg  (Novus Biologicals)

Journal: Non-coding RNA Research

Article Title: Exosomal miRNA-218–5p derived from low-passage dermal papilla cells modulates hair follicle growth and development

doi: 10.1016/j.ncrna.2026.01.004

Figure Lengend Snippet: DPC-Exos transported miR-218-5p from DPCs to HFSCs. (A) Fluorescence imaging of HFSCs after treatment with DiI-labeled DPC-Exos (scale bar = 50 μm). (B) Schematic of the DPC–HFSC co-culture system with fluorescence showing intercellular transfer of exosomal miR-218–5p. (C) MiR-218–5p expression in HFSCs after transfection with Cy3-miR-218–5p (unpaired two-tailed t -test, n = 3). (D) MiR-218–5p expression in HFSCs after DPCs were transfected with siRNA-Drosha (unpaired two-tailed t -test, n = 3). (E) MiR-218–5p expression in HFSCs after DPCs were treated with GW4869 (unpaired two-tailed t -test, n = 3). ∗∗ P < 0.01.

Article Snippet: After three times washing with PBS containing 0.1 % Tween-20 (Solarbio, China, Cat. No. ST825), secondary antibodies Cy3-conjugated Goat Anti-Mouse lgG (Proteintech, China, Cat No. SA00009-1) and Fluorescein (FlTc)-conjugated Goat Anti-Mouse lgG (Proteintech, China, Cat No. SA00003-1) were applied for incubation.

Techniques: Fluorescence, Imaging, Labeling, Co-Culture Assay, Expressing, Transfection, Two Tailed Test